FIELD: agriculture.
SUBSTANCE: invention relates to genetics and breeding of farm animals. The method provides for multiplex amplification of 13 loci of microsatellites of cattle (TGLA126, ILSTS005, ETH185, TGLA122, INRA023, ILSTS006, TGLA227, ETH225, ETH10, BM1818, BM1824, SPS115) using a test system for DNA-expertise of animals, building allelic profiles and subsequent calculation of similarity factor Q. At the same time for determination of genuineness of strain it is not required to previously determine allelic profiles of breeds and using reference populations. Those species are of genuine strain, which have similarity factor Q≥0.75.
EFFECT: method makes it possible to determine genuineness of strain of animals without previous research of the allelic fund of breeds.
1 dwg, 1 ex
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