FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology, more specifically to a kit of synthetic oligonucleotide primers, and can be used forensic identification medicine. The declared kit consists of pairs of primers complementary to respective regions of fifteen microsatellite loci of Y-chromosome which represent pairs of primers one of which is fluorescent-labelled, while the second one is unlabelled with the forward primer for each of the loci bear a fluorescent label on 5'-terminal: TET (4,7,2',7'-tetrachloro-6-carboxyfluorescein) for DYS385a/b, DYS390, DYS391, DYS426 and DYS439; FAM (6-carboxyfluorescein) for DYS392, DYS393, DYS437 and DYS438; and HEX (4,7,2',4',5',7'-hexachloro-6-carboxyfluorescein) for DYS394, DYS388, DYS389I/II and DYS434. The invention also refers to a method for genetic typing for personal identification in the Russian population with the use of the above primers.
EFFECT: invention enables personal identification in the Russian population having higher sensitivity, specificity and accuracy as compared to the existing analogues.
2 cl, 2 dwg
