DEVICE TO EXTRACT NUCLEIC ACIDS Russian patent published in 2013 - IPC C12N15/00 B82B3/00 

FIELD: biotechnologies.

SUBSTANCE: invention is designed for extraction and treatment of DNA and RNA from biological samples - samples with purity suitable for their subsequent analysis by the method of polymerase chain reaction in real time (Real Time PCR). The device comprises a body, test tubes for placement of nucleic acids adsorbed on magnetic particles in each of them and a pestle magnetised perpendicularly. The body is made of non-magnetic material. In the body there are the following components installed as capable to generate rotary non-uniform magnetic field: a horizontally arranged shaft and magnetic cylinders. The shaft comprises blocks of four crossed permanent magnets that repeat along the longitudinal axis of the shaft. The position of each magnet is determined by the angle of rotation of identical poles relative to the first magnet, the second magnet is turned by 90 degrees, the third one - by 270 degrees, the fourth magnet - by 180 degrees. Magnetic cylinders are arranged at both sides of the horizontal shaft. Each of the magnetic cylinders is magnetised perpendicularly as capable of rotation only around this axis, and is arranged in parallel to the test tube wall. Each pair of magnetic cylinders at different sides of the longitudinal axis of the horizontal shaft is arranged as capable of interaction with two crossed permanent magnets of the horizontal shaft, besides, the horizontal shaft is made and placed in the body so that its axis is perpendicular to the axes of magnetic cylinders. The number of pairs of crossed permanent magnets on the shaft is equal to the number of magnetic cylinders arranged at one side from the longitudinal axis of the shaft, at the same time test tubes are arranged in the body in magnetic non-uniform rotary field.

EFFECT: increased efficiency of wear of a coil of magnetic particles entangled with nucleic acids and production of samples with purity suitable for their subsequent analysis by the method of polymerase reaction.

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