NOVEL MUTANT ORNITHINE DECARBOXYLASE PROTEIN AND USE THEREOF Russian patent published in 2018 - IPC C12N9/88 C12N15/60 C12N15/63 C12P13/00 

FIELD: chemistry.

SUBSTANCE: modified ornithine decarboxylase (ODC) protein, having improved putrescine productivity, and its use are disclosed. Isoleucine at position 163 from the N-terminus of ODC having the amino acid sequence presented by SEQ ID NO: 1, is replaced with an amino acid residue that is less than isoleucine, and / or glutamic acid at position 165 from the N-terminus of ODC having the amino acid sequence represented by SEQ ID NO: 1, is replaced with an amino acid residue that is less than glutamic acid. Polynucleotide encoding modified ODC protein, having improved putrescine-convertase activity, is disclosed. Also disclosed are an expression vector comprising said polynucleotide and a transformant transformed with said vector, and a recombinant microorganism having improved putrescine-producing activity by replacing the native ODC protein with said modified ODC protein in a Corynebacterium sp. microorganism having putrescine-producing activity. Versions of the method of producing putrescine are also disclosed.

EFFECT: group of inventions provides putrescine products in high yield.

14 cl, 1 dwg, 9 tbl, 6 ex