METHOD OF DETERMINING CONCENTRATION OF ASCORBIC ACID Russian patent published in 2013 - IPC G01N33/15 

FIELD: chemistry.

SUBSTANCE: invention describes a method of determining concentration or content of ascorbic acid in liquid drug formations, involving preparation of doses of an analysed sample and a standard sample, wherein 0.6 ml of a sodium phosphate buffer (0.05 mol/l, pH 8,3), 0.1 ml of tetramethylene diamine solution (0.05 mol/l) in a sodium salt of ethylenediamine tetraacetic acid (0.2 mmol/l), 0.1 ml of the analysed sample and 0.2 ml of riboflavin solution (0.034 mmol/l) are successively fed into a chemiluminometer cell; the same volume of distilled water is added to the control sample; further, the reaction is initiated by irradiation with a visible light lamp (100 W) at a distance of 20 cm for 60 s; 10 s before irradiation time expires, 0.1 ml of luminol solution (0.25 mmol/l) is added; after irradiation, the cell holder is moved into the operating position in front of the photocathode of a photomultiplier (about 1 s) in a biochemiluminometer and the chemiluminometer flash is detected; concentration of ascorbic acid in the mixture or content thereof is calculated in the same units it was recorded using a formula.

EFFECT: method is characterised by optimum rapidness, easy preparation of samples for analysis and high reproducibility and accuracy.

4 ex, 1 tbl, 1 dwg