FIELD: chemistry.
SUBSTANCE: group of inventions relates to biotechnology, particularly to bioreactors, primarily micro- and mini-bioreactors with an enzyme immobilised on particles and can be used to conduct biotechnological processes in liquid media in pharmaceutical, food and other industries. The microbioreactor has a housing, connecting pipes, pumps for feeding a substrate and auxiliary substances that are connected to said housing by pipes. There is a distribution device and parallel channels in the housing. The channels have a cross-section with a periodically varying length. Each of the channels contains solid particles with an immobilised enzyme. The distribution device consists of a chamber for feeding a substrate and the next one or more chambers for feeding auxiliary substances. There is one or more openings in the side wall of the channels in each chamber for feeding auxiliary substances, wherein channels in the top and bottom parts are equipped with grids, the diameter of openings in which is less than the diameter of the solid particles. Each of the channels can have additional grids which are fitted in sections with the largest area. The method of operating the microbioreactor involves using one or more pumps to feed the substrate and auxiliary substances into the housing with rates that vary periodically over time, wherein the maximum speed of the substrate with auxiliary substances in the wide section of the channels in one period is set higher than the rate of fluidisation of solid particles with the immobilised enzyme, and the minimum speed of the substrate with auxiliary substances in the wide section of the channels in one period is lower than the rate of the onset of fluidisation. Duration of the interval of maximum rate is set such that particles with the immobilised enzyme are able to travel the distance from the bottom to the top grid, and duration of minimum rate is set such that particles with the immobilised enzyme are able to travel the distance from the top to the bottom array. The maximum speed of the substrate with auxiliary substances in the wide section of the channels in one period is set higher than the rate of deposition of solid particles with the immobilised enzyme, and the minimum speed of the substrate with auxiliary substances in the wide section of the channels in one period is set negative.
EFFECT: group of inventions increases efficiency of mass-exchange and bioreactor processes and, as a result, increases efficiency of the apparatus.
5 cl, 6 dwg
