FIELD: biology.
SUBSTANCE: present invention relates to devices for growing tissue cells and microorganisms in null-gravity conditions and can be used in space biotechnology. The device has a cylindrical container with a cover and a device for aerating and moving suspension of the said biological objects, comprising connecting pipes for inlet of aerating gas and outlet of gaseous medium respectively, fitted in the cover of the container, and made with possibility of creating on the surface of the suspension, rotational flow of aerating gas with velocity field of the potential vortex on the periphery of the container and an axial counter-flow in the paraxial area and a 10-2000 Pa pressure drop between the periphery and the centre of the vortex. The inner surface of the container and the bottom are made from material with limiting wetting angle θ=90°, or part of the inner surface on the side of the bottom and the bottom itself are made from material soaked with a liquid substrate with limiting wetting angle θ<90°. The other part of the inner surface of the container, on the side of its cover, is made from material not soaked with liquid substrate with limiting angle θ>90°, where in degrees are values of wetting angles of aqueous substrates on solid surfaces. The inner surface of the container which is not wet, on the side of the cover, can be made in form of a cylindrical insert with a holder, holding it in position, the outer diametre of which corresponds to the inner diametre of the container and is put into the container with possibility of moving along its axis. In an alternative of making the device, the inner surface of the container which is not wet, made in form of a cylindrical insert, is made from Teflon, and the rest of the soaked part of the surface of the container is made from stainless steel.
EFFECT: process of growing cells or microorganisms in microgravity conditions due to provision for stable conditions of the boundary surface between liquid and gaseous phases.
7 cl, 7 dwg, 3 tbl
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Authors
Dates
2009-05-20—Published
2007-12-19—Filed