METHOD OF PREPARATION OF TACROLIMUS BY MICROBIOLOGICAL SYNTHESIS METHOD Russian patent published in 2013 - IPC C12P17/18 C12N1/20 C12R1/465 

FIELD: biotechnology.

SUBSTANCE: method includes preparation of the inoculum of cells Streptomyces sp. MT246 (All-Russian Collection of Microorganisms Ac-2618D). Preparation of the nutrient medium containing one or more sources of carbon, nitrogen, metal ions in the form of soluble salts with fractional feeding of the carbon source during the productive stage. At that, as an additional carbon source the nutrient medium contains rhamnose, and a necessary component of the nutrient medium - MnS0₄ and sorbent from the group of amberlites XAD. Adding of the inoculate of cells Streptomyces sp. MT246 (All-Russian Collection of Microorganisms Ac-2618D) to the nutrient medium together with rhamnose and MnSO₄ in a predetermined ratio and incubating of the medium at pH 7.0-7.5, temperature 25-35°C for 7-10 days. Separation of the residue is carried by centrifugation. The resulting residue is added to ethanol at a predetermined ratio, followed by extraction of tacrolimus at 30 for 2 hours and centrifuging to obtain the extract comprising tacrolimus.

EFFECT: invention enables to increase the yield of tacrolimus.

9 cl, 6 ex