FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology. Disclosed are a strain of actinomycete Streptomyces tsukubensis VKM Ac-2832D, having the ability to produce tacrolimus, and a method of producing tacrolimus by culturing the disclosed strain on a nutrient medium containing starch 75 g/l and glucose 5 g/l, lyophilized corn extract 12.5 g/l and baking yeast 16.6 g/l, L-lysine monohydrochloride 5 g/l, manganese sulphate (II) 0.05 g/l and calcium carbonate 3 g/l, in the presence of highly porous nonionic sorbent Sepabeads SP-207 in amount of 40 g/l of nutrient medium with daily make-up of 9 % sterile solution of fawn dextrin.
EFFECT: invention increases output of the end product and enables to scale the process of producing tacrolimus in industrial conditions.
2 cl, 2 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
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|
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