PRODUCTION METHOD OF COMPLEX FEED PREPARATION WITH LYSIN PRODUCER BASED ON CORYNEBACTERIUM GLUTAMICUM Russian patent published in 2013 - IPC C12N1/20 A23K1/00 C12R1/125 C12R1/10 C12R1/15 C12R1/865 

FIELD: biotechnologies.

SUBSTANCE: liquid culture of strain Corynebacterium glutamicum All-Russian collection of industrial microorganisms B-1959 - lysine producer by means of a deep method and liquid cultures of strains Bacillus subtilis All-Russian collection of industrial microorganisms B-8130, Bacillus subtilis All-Russian collection of industrial microorganisms B-2984, Bacillus subtilis All-Russian collection of industrial microorganisms B-4099 and Bacillus licheniformis All-Russian collection of industrial microorganisms B-4162 are prepared. To liquid culture of strain Corynebacterium glutamicum All-Russian collection of industrial microorganisms B-1959 there added in quantity of 30 l is 35 l of the mixture consisting of liquid cultures Bacillus subtilis All-Russian collection of industrial microorganisms B-8130, Bacillus subtilis All-Russian collection of industrial microorganisms B-2984 and Bacillus subtilis All-Russian collection of industrial microorganisms B-4099, which have been taken in the ratio of 6:6:1 respectively. Common mixture of liquid cultures is applied onto the carrier prepared in advance - sterile exhausted beet pulp treated with cellulolytic ferment and enriched with fermentolysate of Saccharomyces cerevisiae yeast. Then, it is mixed and exposed; after that, solid-phase fermentation is carried out under the specified conditions of restricted oxygen access. Liquid culture Bacillus licheniformis All-Russian collection of industrial microorganisms B-4162 containing at least 5.6×10⁸ CFU/g is added in the amount of 65 l. The mixture is mixed and dried to the humidity of 8-10%. Dry powders of purple echinacea and fruits of holy thistle are added in terms of 20-50 g per 1 kg of the end product. The obtained mixture is stirred and subject to crushing till indiscrete mass is obtained.

EFFECT: invention allows improving feedstuff quality.

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