FIELD: biotechnologies.
SUBSTANCE: medium contains agarose or agar (500 mg), non-ionic detergent (50 mcl), calcium chloride (12.5 mg), 0.05 M Tris-HCl buffer pH 8.3 (up to 100 ml); with that, after medium is hardened, pits are made in it, into which the tested specimen is introduced in the volume of 10-50 mcl per pit; then, medium with the tested specimen is incubated at 37°C during 12 hours and presence or absence of lipolytic activity in the tested specimen is visually determined. At availability of lipolytic activity there observed are mat coronae around the pit; when lipolytic activity is absent, medium around the pit remains clear.
EFFECT: use of the proposed method allows easy, available and economic determination of lipolytic activity of subcellular fractions of bacteria.
4 cl, 6 ex
