METHOD OF DETERMINING HEMOGLOBIN CONCENTRATION IN BIOLOGICAL TISSUES Russian patent published in 2013 - IPC A61B5/1455 

FIELD: medicine.

SUBSTANCE: invention relates to field of laboratory medical analysis, analytical instrument making and can be used for determination of total hemoglobin concentration in biological tissues. Transmission of radiation on tissue into one or more points is performed at wavelengths λ equal 524, 578 and 662 nm or 524, 578 and 773 nm. Signals of diffuse reflection P(L_n,λ) at three or more distances L_n (n=1, 2, 3 …) between points of transmission and registration of radiation are measured. Standard signals r_n(λ)=P(L_n,λ)/P(L₁,λ) and their main components are determined, and total hemoglobin concentration is calculated on the basis of multiple regression equation, connecting it with main components of r_n(λ).

EFFECT: increased accuracy of determination of total hemoglobin concentration in biological tissues due to taking into account presence of oxyhemoglobin, deoxyhemoglobin, methemoglobin and carboxyhemoglobin in them, exclusion of impact of variations of thin upper tissue layer parameters (for instance, skin epidermis) and its diffusing properties, elimination of calibration measurements, as well as reduction of method cost.

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