METHOD TO DETERMINE CONCENTRATIONS OF BIOLOGICAL TISSUE CHROMOPHORES Russian patent published in 2014 - IPC G01N21/31 A61B5/1455 

FIELD: measurement equipment.

SUBSTANCE: invention related to the field of laboratory medical analysis, analytic instrument-making. The method consists in the fact that parameters of conversion for a system of image recording with specified equipment functions, including the main components of normalised spectral signals r(Λ_k)=V(Λ_k)/V(Λ₀), where Λ₀ - reference spectral section, and coefficients of non-linear multiple regressions between concentrations of chromophores C_q and normalised signals r(Λ_k) or their projections to space of main components, they determine by means of simulation of radiation transfer into tissues with account of characteristics of the used system of recording and possible ranges of variations of structural and biochemical parameters of the tissue. At the same time the tissue is illuminated with a linearly polarised radiation, a multispectral image is recorded V_┴(x, y, Λ_k) with polarisation, orthogonal to polarisation of illuminating radiation, additionally they record a multispectral polarised image V_┴(x, y, Λ_k) with absence of tissue illumination. For each point x, y they produce normalised spectral signals r(Λ_k) by normalisation of layers of a differential multispectral image V_┴(x, y, Λ_k)-V_┴(x, y, Λ_k) to a differential reference layer V_┴(x, y, Λ₀)-ν_┴(x, y, Λ₀), and concentration of chromophores C_q(x, y) in each point x, y is determined in accordance with corresponding normalised spectral signals using non-linear multiple regressions between C_q and r(Λ_k) or between C_q and projections r(Λ_k) to the space of main components.

EFFECT: invention provides for increased accuracy of production of 2D distributions of biological tissue chromophore concentration.

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