METHOD FOR DETERMINING ADHESIVE PROPERTIES OF BACTERIA OF ENTEROCOCCUS FAMILY BY MEANS OF CaCo-2 CELL LINE Russian patent published in 2013 - IPC C12Q1/02 G01N33/53 C12N1/20 

Abstract RU 2501861 C1

FIELD: biotechnologies.

SUBSTANCE: method involves preparation of bacterial suspension, separation of the obtained biomass of bacteria by centrifugation, dilution of the obtained biomass in physiological solution, preparation of monolayer of CaCo-2 cells, introduction of bacterial culture, cultivation of cells, flushing with physical solution, removal of monolayer with bacterial cells and counting of the amount of bacterial cells related to 1000 cells of CaCo-2; with that, bacteria refer to highly-adhesive ones if the number of bonded cells is 1010 to 3000, to average-adhesive ones of 210 to 1000, and to low-adhesive ones of 0 to 200.

EFFECT: improvement of the method.

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RU 2 501 861 C1

Authors

Mashentseva Natalija Gennadievna

Nguen Tkhi Min' Kkhan'

Dates

2013-12-20Published

2012-05-12Filed