FIELD: veterinary science.
SUBSTANCE: alleged invention concerns veterinary protozoology. There is disclosed method for preparing anaplama antigen for serm diagnostics in animals, including infection with cattle anaplasmosis agent, centrifugation anaplasma recovery from blood, evaluation of activity in "РДСК" differing is plasma prepared in centrifugation and recovered from blood of infected animals, is cleared from deposited anaplasmas, processed with 20-25% polyethylene glycol 6000 taken in the same ratio as blood plasma; further the prepared mixture is kept at room temperature for 13-20 min, centrifugated again at 6000 rpm for 16-25 min; then supernatant fluid is removed, and the produced sediment is used as anaplasma exoantigen in serum tests with its activity to be 95-97%.
EFFECT: invention provides preparing high active anaplasma antigen.
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Authors
Dates
2009-11-10—Published
2008-03-14—Filed