WAY OF RECEPTION OF BRUCELLOUS ANTIGEN FROM STRAIN BRUCELLA ABORTUS 19 FOR MANUFACTURING OF UNIFORM BRUCELLOUS ANTIGEN FOR AA (AGGLUTINATION ASSAY), CFR (COMPLEMENT FIXATION REACTION) AND LCFR (LONG-TERM COMPLEMENT FIXATION REACTION), BRUCELLOUS ANTIGEN FOR ROSE BENGAL ASSAY (RBA) AND BRUCELLOUS ANTIGEN FOR RING TEST (RT) WITH MILK, WAY OF MANUFACTURING OF BRUCELLOUS DIAGNOSTIC SERUM AND DIAGNOSTIC SETS Russian patent published in 2009 - IPC A61K39/10 A61K39/40 A61P31/04 

Abstract RU 2361610 C1

FIELD: medicine, veterinary science.

SUBSTANCE: group of inventions concerns veterinary science and biotechnology area. The way of reception of a brucellous antigen from the Brucella abortus 19 strain for manufacturing of a uniform brucellous antigen for AA, CFR and LCFR includes reception of an inoculum and propagation of brucellas in deep conditions on a liquid nutrient medium with regulation of level of partial pressure of oxygen dissolved in a culture liquid throughout all propagation process, separation and concentration of evolved bacteriemic cells using ultrafiltration and their inactivation using warming. Thus brucellas propagation in deep conditions on a liquid nutrient medium with regulation of level of partial pressure of oxygen dissolved in a culture liquid throughout all propagation process is performed in a following regimen: temperature 37±0.5°C, pH 6.9-7.2 aeration from 1 hour till 3 hour - 20-25 pO2, from 3 hour till 6 hour - 25-40 pO2, from 6 hour till 15 hour - 40-45 pO2 and from 15 hour till 16-18 hour - 30-35 pO2. Separation and concentration of the evolved bacteriemic cells by ultrafiltration is carried out with use of membranous cartridges. The concentrate of an antigen received by given way is used also for reception of an antigen for Rose Bengal Assay (RBA), for reception of a brucellous antigen for ring test (RT) with milk and reception of diagnostic brucellous serum which, together with blood serum of healthy livestock, make a part of sets for brucellosis diagnostics. The group of inventions allows to raise efficiency of process of brucellosis diagnostics and to improve control at serologic examination of animals.

EFFECT: rising of efficiency of process of brucellosis diagnostics and improvement of control at serologic examination of animals

22 cl, 11 ex

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Authors

Sobolev Viktor Vasil'Evich

Mel'Nik Nikolaj Vasil'Evich

Skljarov Oleg Dmitrievich

Trojnin Anatolij Serafimovich

Zenov Nikolaj Ivanovich

Klimanov Arkadij Ivanovich

Shumilov Konstantin Vasil'Evich

Litenkova Irina Jur'Evna

Rakhmanin Pavel Petrovich

Krjukov Sergej Veniaminovich

Trenev Vasilij Nikolaevich

Solov'Ev Boris Vasil'Evich

Balashov Vladimir Grigor'Evich

Dates

2009-07-20Published

2008-03-20Filed