FIELD: medicine.
SUBSTANCE: method for preparing a corneal biotransplant involving the application on a matrix representing a polymer film of non-modified nanostructured hyaluronic acid, corneal cells represented by an autogenous mixed cell culture containing epithelial corneal progenitors and corneal fibroblasts taken in specific proportions and culture on a medium containing the cultural concentration of the antibiotics.
EFFECT: using the method described above leads to reducing the length of culture up to 9-12 days and maintaining the additional prolonged maintenance of biotransplant cell migration and proliferation.
2 dwg, 1 tbl
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Authors
Dates
2014-01-27—Published
2011-09-13—Filed