FIELD: medicine.
SUBSTANCE: invention refers to a method for producing a product of reaction catalysed by a protein showing 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-b-isoleucine or its salt and 4-hydroxy-b-proline or its salt with using a bacterium of a genus selected from a group consisting of Escherichia, Corynebacterium, Arthrobacter, Aspergillus and Bacillus, transformed by a DNA fragment containing a gene coding the protein showing 2-oxoglutarate-dependent enzyme activity with said bacterium modified so that: the gene coding 2-oxoglutarate dehydrogenase is inactivated, or the gene coding 2-oxoglutarate dehydrogenase, and the gene coding aminotransferase with branched-chain amino acid are inactivated.
EFFECT: invention enables high-effective production of said compounds and their salts.
14 cl, 6 dwg, 9 tbl, 9 ex
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