RECOMBINANT POLYPEPTIDE A2 SELECTIVELY BINDING HSA, RECOMBINANT DNA pa2 CODING HSA-BINDING PART OF POLYPEPTIDE A2, PRODUCER THEREOF - RECOMBINANT STRAIN OF Escherichia coli M15-A2, CONTAINING RECOMBINANT PLASMID DNA pQE 32-pa2, FACILITATING PRODUCTION OF POLYPEPTIDE A2 AND USE OF POLYPEPTIDE A2 FOR DIAGNOSIS OF MICROALBUMINURIA AND SEPARATING HSA FROM BLOOD SERUM Russian patent published in 2014 - IPC C07K14/315 C12N15/31 C12N15/70 C12N1/21 G01N33/493 G01N33/53 

FIELD: chemistry.

SUBSTANCE: invention relates to microbiology and molecular genetics and pertains to a recombinant polypeptide A2, DNA coding said polypeptide, a strain which produces polypeptide A2 and methods of using such a recombinant polypeptide. The disclosed recombinant polypeptide A2 is characterised by an amino acid sequence of 346 amino acids in which the first 13 amino acids are coded by the plasmid sequence pQE 32 and are covalently bonded with the next 333 amino acids which are coded by a sequence of the HSA-binding fragment of chromosomal DNA of the strain DG 13 of streptococci of the group G-CFG.

EFFECT: group of inventions can be used in diagnosis, eg, when making a test system for determining microalbuminuria based on a laboratory criterion of the preclinical phase of diabetic nephropathy, as well as a reagent for separating human serum albumin by affinity chromatography and for freeing serum from HAS, which enables to determine other proteins present in the serum in lower concentrations.

9 cl, 11 dwg, 1 tbl, 9 ex