FIELD: chemistry.
SUBSTANCE: inventions relate to field of molecular biology and deal with recombinant DNA pA4, recombinant plasmid DNA pQE 30-A4, Esherichia coli strain M15-A4, recombinant polypeptide A4, possessing ability to selectively bind human serum albumin (HSA), affine sorbent, containing such polypeptide, affine combined sorbent and methods for successive removal of HSA and IgG from blood serum. Characterised affine combines sorbent is obtained by mixing affine sorbent, containing said recombinant polypeptide A4, and analogous affine sorbent, in which known IgG-binding polypeptide is used as ligand.
EFFECT: claimed inventions can be used in medical practice to deplete blood serum of two proteins, albumin (HSA) and immunoglobulin G (IgG), present in it in high concentrations Removal of two major proteins from blood serum will make it possible to identify other proteins, present in serum in lower concentrations.
8 cl, 9 dwg, 7 ex, 1 tbl
| Title |
Year |
Author |
Number |
| RECOMBINANT DNA OF pG4223 AND PLASMID DNA PQE 30-pG4223, STRAIN OF ESCHERICHIA COLI M 15-G4223 WHICH ENABLE TO OBTAIN G4223 POLYPEPTIDE SELECTIVELY BINDING IgG, AND USE THEREOF IN AFFINE CHROMATOGRAPHY TO ISOLATE IgG |
2017 |
- Gupalova Tatyana Vitalievna
- Bormotova Elena Alekseevna
|
RU2715672C2 |
| RECOMBINANT DNA pA3, RECOMBINANT DNA pQE 30-pA3 PROVIDING PRODUCING POLYPEPTIDE A3, STRAIN E. coli M 15-A3 TRANSFORMED BY RECOMBINANT PLASMID DNA pQE 30-pA3 AND EXPRESSING RECOMBINANT POLYPEPTIDE A3, RECOMBINANT POLYPEPTIDE A3 POSSESSING ABILITY TO BIND HUMAN SERUM ALBUMIN, AND RFA TEST SYSTEM FOR QUALITATIVE DETECTION OF MICROALBUMINURIA, TEST SYSTEM FOR QUANTITATIVE DETERMINATION OF MICROALBUMINURIA |
2013 |
- Gupalova Tat'Jana Vitalievna
- Bormotova Elena Alekseevna
|
RU2550255C2 |
| RECOMBINANT POLYPEPTIDE A2 SELECTIVELY BINDING HSA, RECOMBINANT DNA pa2 CODING HSA-BINDING PART OF POLYPEPTIDE A2, PRODUCER THEREOF - RECOMBINANT STRAIN OF Escherichia coli M15-A2, CONTAINING RECOMBINANT PLASMID DNA pQE 32-pa2, FACILITATING PRODUCTION OF POLYPEPTIDE A2 AND USE OF POLYPEPTIDE A2 FOR DIAGNOSIS OF MICROALBUMINURIA AND SEPARATING HSA FROM BLOOD SERUM |
2011 |
- Gupalova Tat'Jana Vitalievna
- Bormotova Elena Alekseevna
- Totoljan Artem Akopovich
|
RU2506271C2 |
| RECOMBINANT GM PROTEIN CAPABLE OF BINDING Α2-MACROGLOBULIN AND APPLICATION THEREOF AS A LIGAND IN AFFINITY CHROMATOGRAPHY FOR EXTRACTING Α2-MACROGLOBULIN FROM HUMAN BLOOD SERUM |
2019 |
- Gupalova Tatyana Vitalievna
- Bormotova Elena Alekseevna
|
RU2758604C2 |
| RECOMBINANT POLYPEPTIDE StV POSSESSING PROTECTIVE PROPERTIES IN RELATION TO Streptococcus agalactiae, RECOMBINANT DNA StV, ITS CODING AND RECOMBINANT STRAIN Escherichia coli M15-StV CONTAINING RECOMBINANT PLASMID PQE-StV |
2012 |
- Suvorov Aleksandr Nikolaevich
- Gupalova Tat'Jana Vital'Evna
- Kuleshevich Evgenija Vladimirovna
- Grabovskaja Kornelija Borisovna
- Leont'Eva Galina Fedorovna
|
RU2542483C2 |
| RECOMBINANT DNA PROVIDING PRODUCTION OF POLYPEPTIDES P6, P7, P8 EXHIBITING PROTECTIVE PROPERTIES AGAINST STREPTOCOCCUS AGALACTIAE AND SELECTIVELY CONNECTING IgA |
2008 |
- Suvorov Aleksandr Nikolaevich
- Ustinovich Irina Anatol'Evna
- Meringova Ljudmila Fedorovna
- Leont'Eva Galina Fedorovna
- Grabovskaja Kornelija Borisovna
- Korzhueva Aleksandra Sergeevna
|
RU2387715C2 |
| RECOMBINANT DNA ENSURING PRODUCTION OF RECOMBINANT PROTEIN PBI EXPRESSING PROTECTIVE PROPERTIES IN RELATION TO STREPTOCOCCUS PYOGENES AND STREPTOCOCCUS AGALACTIAE |
2008 |
- Koroleva Irina Vladimirovna
- Duplik Nadezhda Vladlenovna
- Suvorov Aleksandr Nikolaevich
|
RU2378374C2 |
| RECOMBINANT PLASMID DNA OF PQE-30_P36GP12_GP57, PROVIDING SYNTHESIS OF RECOMBINANT PROTEIN P36GP12 IN CELLS OF ESCHERICHIA COLI, A STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF RECOMBINANT PROTEIN P36GP12, RECOMBINANT PROTEIN P36GP12, CAPABLE TO BIND LIPOPOLYSACCHARIDES ESCHERICHIA COLI |
2019 |
- Bokovaya Olga Vasilevna
- Bajkov Ivan Konstantinovich
- Morozova Vera Vitalevna
- Kozlova Yuliya Nikolaevna
- Tikunova Nina Viktorovna
|
RU2707922C1 |
| RECOMBINANT DNA ENSURING PRODUCTION OF THE RECOMBINANT PROTEIN COV1 EXHIBITING IMMUNOGENIC PROPERTIES AGAINST SARS-COV-2 VIRUS |
2021 |
- Koroleva Irina Vladimirovna
- Suvorov Aleksandr Nikolaevich
- Leonteva Galina Fedorovna
- Kramskaya Tatyana Anatolevna
- Desheva Yuliya Andreevna
- Shvedova Tamara Nikolaevna
|
RU2776484C1 |
| RECOMBINANT PLASMID DNA PPINS07 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
1999 |
- Korobko V.G.
- Boldyreva E.F.
- Shingarova L.N.
- Vul'Fson A.N.
- Kostromina T.I.
- Miroshnikov A.I.
- Gavrikov V.G.
- Kalinin Ju.T.
- Stepanov A.V.
|
RU2144957C1 |
