METHOD OF CREATION OF TRANSGENIC PLANTS WITH HIGH LEVEL OF EXPRESSION OF TRANSGENIC PROTEINS Russian patent published in 2014 - IPC A01H1/00 

Abstract RU 2507736 C2

FIELD: agriculture.

SUBSTANCE: invention relates to the field of biochemistry and in particular to the method of creation of transgenic plant lines that produce protein with high level of expression, comprising transformation of plants with an expression vector comprising the plasmid comprising the gene encoding β-glucuronidase, 35S promoter, nos transcription terminator. At that the transcription terminator is inserted above the promoter of the gene, which has the ability to break effectively the genomic transcripts in the plant genome and to protect effectively the expression of the transgene in the plant genome from the subsequent repressions.

EFFECT: invention enables to create transgenic plant lines that produce protein with high levels of expression.

1 dwg, 3 tbl, 5 ex

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RU 2 507 736 C2

Authors

Maksimenko Oksana Gennad'Evna

Dolgova Anna Sergeevna

Bonchuk Artem Nikolaevich

Tikhonov Maksim Vasil'Evich

Gasanov Nizami Badrudinovich

Zarajskij Evgenij Il'Ich

Dolgov Sergej Vladimirovich

Georgiev Pavel Georgievich

Dates

2014-02-27Published

2011-01-11Filed