FIELD: medicine.
SUBSTANCE: mammal cells are transformed by an expression vector containing a reporter gene, a cytomegalovirus promotor and a transcription terminator built above a sequence of the cytomegalovirus promotor.
EFFECT: method can be used for producing mammal cell cultures with a high and stable level of target protein lifelength for large-scale production of recombinant proteins which can be used for the medical and research purposes.
1 dwg, 2 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR CREATING TRANSGENIC ANIMALS WITH STABLE AND HIGH TARGET PROTEIN EXPRESSION IN MILK | 2012 |
|
RU2525712C2 |
| METHOD OF CREATION OF TRANSGENIC PLANTS WITH HIGH LEVEL OF EXPRESSION OF TRANSGENIC PROTEINS | 2011 |
|
RU2507736C2 |
| IMPROVED TRANSGENE EXPRESSION AND PROCESSING | 2014 |
|
RU2808756C1 |
| RECOMBINANT PLASMID DNA phPGK-eGFP-CATTCGCC-CMV-mCherry, PROVIDING HIGH EXPRESSION OF REPORTER PROTEIN eGFP IN CULTURED CHINESE HAMSTER OVARY CELLS | 2023 |
|
RU2822596C1 |
| EXPRESSION PLASMID VECTOR OF MONOCISTRONIC EXPRESSION OF RECOMBINANT PROTEINS IN MAMMALIAN CELLS, CELL LINE OF MAMMALS-PRODUCERS OF RECOMBINANT PROTEIN, METHOD OF OBTAINING RECOMBINANT PROTEIN | 2013 |
|
RU2552170C2 |
| METHOD FOR DETECTING REGULATORY ELEMENTS OF GENOME AND GENETIC CONSTRUCT FOR USE IN SAID METHOD | 2023 |
|
RU2822825C1 |
| EXPRESSION SYSTEM FOR INCREASING GENE EXPRESSION LEVEL, NUCLEIC ACID MOLECULE, TRANSGENIC ANIMAL AND KIT | 2007 |
|
RU2469089C2 |
| NON-TOXIC VECTORS ON HSV BASIS FOR APPLICATIONS IN EFFECTIVE GENE DELIVERY AND COMPLEMENTING CELLS FOR THEIR PRODUCTION | 2014 |
|
RU2714259C2 |
| EXPRESSION VECTOR FOR TRANSGENIC INTRODUCTION INTO CELLS AND TISSUES OF MAMMALS WITH REGULATED NON-VIRAL PROMOTOR | 2011 |
|
RU2495128C2 |
| METHOD OF IDENTIFICATION OF ELEMENTS HAVING ABILITY TO TERMINATE TRANSCRIPTS | 2009 |
|
RU2476597C2 |
