NOVEL BNP (1-32) EPITOPE AND ANTIBODIES DIRECTED AGAINST SAID EPITOPE Russian patent published in 2014 - IPC C07K14/58 C12N15/02 C07K16/18 C12N5/16 G01N33/53 

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry, particularly a polypeptide bearing a human BNP(1-32) epitope, for producing ligands that are directed against the human BNP(1-32) or human proBNP(1-108), where said polypeptide has the formula α1-R₁-X₁-FGRKMDR-X₂-R₂-α₂. Disclosed is use of said polypeptide to produce ligands directed against the human BNP(1-32) or human proBNP(1-108) and for producing a hybridoma which secretes a monoclonal antibody directed against the human BNP(1-32) or human proBNP(1-108). Disclosed is a method of producing a hybridoma which secretes a monoclonal antibody directed against the human BNP(1-32) or human proBNP(1-108), as well as the obtained hybridoma. Disclosed is a ligand which is specific to an epitope with the sequence FGRKMDR, as well as use thereof to detect human BNP(1-32) or human proBNP(1-108) in a biological sample. Disclosed are methods of detecting human BNP(1-32) or a human proBNP(1-108) derivative in a biological sample, a method for in vitro diagnosis, prediction, risk stratification or subsequent observation of long-term results of cardiac and/or vascular pathology in an individual, as well as a method for in vitro diagnosis of stroke in an individual using said ligand. Disclosed is a multi-epitope calibrator designed to obtain calibration curves for analysis of BNP(1-32), proBNP(1-108), as well as a kit for detecting human BNP(1-32) or human proBNP(1-108).

EFFECT: invention enables to efficiently detect cardiac and/or vascular pathologies in an individual.

15 cl, 17 dwg, 12 tbl, 18 ex