PHOTOBIOCATALYST FOR HYDROGEN FORMATION AND PHOTOCATALYTIC METHOD OF OBTAINING HYDROGEN Russian patent published in 2014 - IPC C12N9/00 C12N11/14 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to biotechnology. Claimed is photobiocatalyst, which includes hydrogenase, immobilised in amount not less than 0.1 nmol per cm² on nanostructured mesoporous TiO₂ film. Mesoporous film is prepared from TiO₂ nanocrystals with size from 15 to 25 nm with specific surface 50-100 m²/g. Together with hydrogenase enzyme on titanium dioxide fim mobilized is photosensibiliser FS1 - pigment-protein complex of photosystem 1 in amount 0.01-0.04 nmol FS per 1 cm². Obtained TiO₂ film is 4-8 mcm thick, has specific surface 50-65 m²/g, pores with average radius 11.5 nm and specific volume 0.50-0.65 cm³/g. Also claimed is photocatalytic method of obtaining hydrogen in anaerobic conditions with application of claimed photobiocatalyst with illumination with light with λ=490-750 nm in presence of organic electron donor and electron carrier.

EFFECT: inventions ensure increased stability of pigment-protein complex of photosystem one and hydrogenase, as well as make it possible to obtain hydrogen under action of visible light with high speed.

3 cl, 3 dwg, 4 ex