TITANIUM DIOXIDE NANOCOMPOSITES FOR INTRACELLULAR VIRAL GENOME INACTIVATION, METHOD FOR MAKING THEM Russian patent published in 2012 - IPC C12N15/11 B82B3/00 A61K48/00 

FIELD: medicine.

SUBSTANCE: there are offered nanocomposites showing antiviral activity and used for intracellular viral genome inactivation. Said nanocomposites consists of titanium dioxide nanoparticles whereon polyamine oligonucleotide derivatives (PA-oligo) are immobilised. Polyamines contain 3 to 1000 aminogroups in a molecule, and preferentially represent polylysine, polyethyleneimine and spermine. The titanium dioxide nanoparticles can be presented both in amorphous, and in crystalline state (anatase, brookite). Said nanocomposites can penetrate into cells by common endocytosis without electroporation and other methods disturbing cell membranes. Besides, what is offered is a method for making titanium dioxide nanocomposites whereon conjugated oligonucleotides and linker are immobilised. The method provides making conjugated oligonucleotides and polyamines with yield 90-100%.

EFFECT: invention considerably simplifies a process for making the nanocomposites and maintained high immobilisation density of conjugated oligonucleotides on the titanium dioxide nanoparticles with higher strength of the surface binding.

12 cl, 5 dwg, 2 tbl, 32 ex