PHOTOBIOCATALYST FOR PRODUCING REDUCED FORMS OF NICOTINAMIDE COENZYMES NADH OR NADPH AND PHOTOCATALYTIC METHOD FOR PRODUCING NADH OR NADPH Russian patent published in 2011 - IPC C12N11/14 C12P19/36 C01G23/47 

FIELD: medicine.

SUBSTANCE: offered is a photobiocatalyst for producing reduced forms of nicotinamide coenzymes NADH or NADPH. The photobiocatalyst represents ferredoxin-NADP-reductase immobilised on a surface of nanopatterned mesoporous titanium dioxide TiO₂ film in amount 0.8-1.24 nmole in 1 cm² of the film surface. The film is prepared of nanocrystalline titanium dioxide of size 15-25 nm and specific surface 50-100 m²/g on a glass substrate or on a glass substrate with a an electrically conductive composition. Also, offered is a photocatalytic method for producing the reduced forms of nicotinamide coenzymes NADH or NADPH with using the photobiocatalyst of ferredoxin-NADP-reductase immobilised on the surface of nanopatterned mesoporous titanium dioxide TiO₂ film. The photobiocatalyst allows high speed and specific NADH or NADPH production of NAD⁺ or NADP⁺ under exposure to light.

EFFECT: method for producing reduced forms of nicotinamide coenzymes NADH or NADPH with using the prepared photobiocatalyst provides easy reaction product separation from an enzyme and a semiconductor, and multiple use of the photobiocatalyst.

4 cl, 5 dwg, 4 ex