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2 512 076

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IPC

C12P33/16(2006-01-01)

C07J1/00(2006-01-01)

C12N1/20(2006-01-01)

C12R1/32(2006-01-01)

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Application

2012148620/10, 2012-11-16

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Start date

2012-11-16

(22)

Actual filing date

2012-11-16

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Published

2014-04-10

(72)

Inventor

Kazantsev Alexey VitalievichSavinova Tatyana StepanovnaLukashev Nikolay VadimovichDesyatkin Viktor GrigorievichDovbnya Dmitry VladimirovichKhomutov Sergei MikhailovichSukhodolskaya Galina ViktorovnaShutov Andrei AnatolievichDonova Marina ViktorovnaEgorova Olga ValerievnaSurovtsev Viktor Vasilievich

(73)

Holder

Rossijskaja Federatsija, Ot Imeni Kotoroj Vystupaet Ministerstvo Promyshlennosti I Torgovli Rossijskoj Federatsii

METHOD OF OBTAINING ANDROST-4,9(11)-DIEN-3,17-DIONE FROM PHYTOSTEROL Russian patent published in 2014 - IPC C12P33/16 C07J1/00 C12N1/20 C12R1/32

Abstract RU 2512076 C1

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. Claimed is method of obtaining androst-4,9(11)-dien-3,17-dione from phytosterol. Microbiological oxidative elimination of side chain at atom C¹⁷ with formation of 9α-hydroxyandrost-4-en-3,17-dione is performed. Biomass is separated. 9α-hydroxyandrost-4-en-3,17-dione is extracted from clarified cultural liquid with aprotic organic solvent, selected from aromatic hydrocarbons or organochlorine hydrocarbons. After that, reaction of 9α-hydroxygroup of 9α- hydroxyandrost-4-en-3,17-dione dehydration is carried out in obtained extract. As dehydration agent applied is mineral acid, which contains water and is selected from group, which includes orthophosphoric, pyrophosphoric and chloric acids. Mineral acid is applied in quantity from 1 to 10 mol per 1 mol of 9α- hydroxyandrost-4-en-3,17-dione. In the process of dehydration reaction removal of excessive water is carried out either in presence of effective quantity of pyrophosphoric acid or by azeotropic distillation.

EFFECT: invention makes it possible to intensify dehydration process with application of smaller quantity of mineral acid and exclude side product formation.

11 cl, 1 tbl, 1 ex

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RU 2 512 076 C1

Authors

Kazantsev Alexey Vitalievich

Savinova Tatyana Stepanovna

Lukashev Nikolay Vadimovich

Desyatkin Viktor Grigorievich

Dovbnya Dmitry Vladimirovich

Khomutov Sergei Mikhailovich

Sukhodolskaya Galina Viktorovna

Shutov Andrei Anatolievich

Donova Marina Viktorovna

Egorova Olga Valerievna

Surovtsev Viktor Vasilievich

Dates

2014-04-10—Published

2012-11-16—Filed