METHOD FOR ENZYMATIC SACCHARIFICATION OF LIGNOCELLULOSE MATERIALS Russian patent published in 2014 - IPC C13K1/02 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and enables to obtain soluble carbohydrates in monomer form from lignocellulose materials. The method involves pretreatment of lignocellulose material with lignin content of 3-35% and mechanical activation in planetary, roller, vibration or vibration-centrifugal type mechanical activators. Enzyme preparations with hydrolysing activity with respect to cellulose, hemicellulose, starch and proteins are then added with concentration of the enzyme preparations of 0.5-10 wt% and concentration of the solid phase of 10-40 wt%. Enzymatic hydrolysis is carried out at temperature of 50-65ºC for 1-6 days to obtain a carbohydrate solution. Every 0.5-5 hours, the gel-like oligosaccharide layer is removed from the reacting lignocellulose particles.

EFFECT: invention speeds up saccharification of lignocellulose materials without using aggressive explosive and flammable, environmentally unfriendly reagents.

4 cl, 1 dwg