METHOD OF EXTRACTING BIOLOGICALLY ACTIVE SUBSTANCES FROM BIOMASS OF UNICELLULAR ALGAE OF CHLORELLA SPECIES Russian patent published in 2012 - IPC C12N1/12 A23K1/00 A61K8/00 

FIELD: process engineering.

SUBSTANCE: biomass of Chlorella species unicellular algae dried to 10%-humidity is activated by activator of planetary, vibratory or vibratory-rotary types that allows acceleration of grinding bodies of 60-400 m/s², time of stay in activation zone making 0.5-10 minutes. Ground biomass is suspended in organic solvent, i.e. gasoline or ethyl alcohol, added in extraction in amount of 5-7 litres per 1 kg of dry biomass, and extracted at room temperature for 3-5 hours. Produced extract is filtered to produce soluble and insoluble portions to be dried to produce dry lipid-pigment complex. Extract dried insoluble fraction is mixed with enzymatic agents ''Cellulox-A'' and '' Protocubtillin r3x'' or mixes thereof, and subjected to hydrolysis for 4-8 hours at pH 4-6, and 50-65°C. Note here that enzymic preparations are added to extract insoluble fraction in amount of 0.5-10 wt %.

EFFECT: enzymic hydrolysate separated by centrifugation into soluble and insoluble fractions, and dried to yield dry products.

3 cl, 4 ex