METHOD OF DETERMINING MYCOTOXINS IN ANIMAL AND VEGETABLE PRODUCTS Russian patent published in 2014 - IPC G01N33/02 G01N30/02 

FIELD: chemistry.

SUBSTANCE: disclosed is an express, safe and cheap method of determining mycotoxins in animal and vegetable products. Determination is carried out from 2 g of a sample, the QuEChERS purified extract is divided into three portions of 2 ml each and 300 mcl of chloroform is used as a dispersant in dispersion liquid-liquid micro-extraction. The obtained extracts are collected in micro-vials. The solvent is evaporated. The residue in the first and third micro-vials is dissolved in 50 mcl acetonitrile and the residue in the second micro-vial is dissolved in 50 mcl hexane. Aflatoxins (B1, B2, G1, G2), zearalenone and ochratoxin A are determined in the first micro-vial by HPLC with a fluorimetric detector. Trichothecene mycotoxins (deoxynivalenol, nivalenol, HT-2, T-2, diacetoxyscirpenol, 13-, 15-acetyl deoxynivalenol), penicidin, ochratoxin A and zearalenone are determined in the second micro-vial by gas-liquid chromatography with an electron capture detector. Penicidin and zearalenone are determined in the third micro-vial by HPLC with a donor-matrix detector. The duration of determining mycotoxins is 1.5-2 hours when operating with three chromatographs simultaneously. Sample preparation requires 10.1 ml aceonitrile, 0.9 ml chloroform and 0.05 ml hexane.

EFFECT: using different types of chromatography to determine penicidin, zearalenone and ochratoxin A enables to obtain more reliable analysis results.

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