MICROBIOLOGICAL METHOD OF OBTAINING 1,2-PROPANEDIOLE Russian patent published in 2014 - IPC C12N1/21 C07C31/20 C12R1/19 

FIELD: chemistry.

SUBSTANCE: propanediol-oxydoreductase coding gene is introduced into an E.coli cell, which makes it possible to produce high levels of 1,2-propanediol, in fact, without 1,3-propanediol, with growing on glycerol as the only carbon source. Genes of glycerol dehydrogenase (gldA), dihydroxyacetone kinase (dhaK) and/or methylglyoxal synthase (mgsA) can be additionally inserted to express the said enzymes together with propanediol-oxydoreductase (fucO), as well as a gene of glycerol dehydratase or aldo-keto reductase in order to express the said glycerol dehydratase or aldo-keto reductase together with propanediol-oxydoreductase. The E.coli cell, transformed by the propanediol-oxydoreductase coding gene, can be defective in arabinose, methylglyoxal and/or dihydroxyacetonephosphate metabolism.

EFFECT: invention relates to the field of genetic engineering and can be used for recombinant production of 1,2-propanediol (1,2-PD).

9 cl, 4 dwg, 9 tbl, 3 ex