METHOD OF PRODUCTION OF PLANTS-REGENERANTS OF STRAWBERRIES (IN VITRO) Russian patent published in 2014 - IPC A01H4/00 

Abstract RU 2516341 C2

FIELD: agriculture.

SUBSTANCE: invention relates to the field of biotechnology. The invention provides a process for production of plants-regenerants of strawberry in vitro, comprising administering of explants to the culture, propagation and rooting of the newly formed shoots in vitro, where the fragments of flower disk and flower-stalk of the strawberry flowers are used as explants, taken in the bud stage, which are washed under flowing water for 15-25 minutes, sterilised with 0.1% mercuric chloride solution for 10 minutes, then washed three times with sterile distilled water, the flower disk is released from the sepals and petals, cut into pieces of 5×5 mm and the cut is placed on a culture medium, the flower-stalk is separated from the flower, a piece of 5 mm long is cut and placed also on the nutrient medium.

EFFECT: invention enables to obtain a larger number of plants-regenerants from one explant.

1 tbl, 2 dwg

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RU 2 516 341 C2

Authors

Tikhomirova Ljudmila Ivanovna

Dates

2014-05-20Published

2012-04-26Filed