FIELD: agriculture.
SUBSTANCE: invention relates to agricultural biotechnology. The invention is a method of reproduction of tea tick trefoil, when the matured sterilized seeds of tea tick trefoil (Hedysarum theinum Krasnob) are planted in the Murashige-Skoog culture medium for insertion into tissue of the culture, in 20-30 days the developed shoots are transplanted to the MS medium of reproduction, then the formed conglomerates of microshoots are divided and transplanted to the media comprising 1.0 BAP + L-glutamine and adenine sulphate 100 mg/l. The plants-regenerants are reproduced, rooted and adapted to non-sterile conditions.
EFFECT: invention enables to improve the efficiency of the process, replicability, adaptation under conditions of hydroponics, and to obtain standard seedlings of tea tick trefoil.
4 dwg, 1 tbl
Title | Year | Author | Number |
---|---|---|---|
CULTURE OF ROOT STRAIN Hed th (Hedysarum theinum Krasnob) - PRODUCER OF ISOFLAVONS | 2008 |
|
RU2360964C1 |
METHOD FOR OBTAINING CALLUS CULTURE HEDYSARUM ALPINUM L. | 2022 |
|
RU2787746C1 |
METHOD OF PRODUCTION OF WHITE CINQUEFOIL (Potentilla alba) | 2012 |
|
RU2525676C2 |
METHOD OF OBTAINING PLANT-REGENERATES OF IRIS ENSATA (I. ENSATA THUNB.) IN VITRO | 2011 |
|
RU2481766C1 |
METHOD OF KARELIAN BIRCH HYBRIDS CLONAL MICROMULTIPLICATION | 0 |
|
SU1752284A1 |
METHOD TO PRODUCE MEDICINAL VEGETABLE STOCK OF Potentilla alba L. UNDER CONDITIONS OF HYDROPONIC CULTURE | 2014 |
|
RU2570623C1 |
METHOD OF MICROCLONAL PROPAGATION OF SIBERIAN FLAG-LEAF (I.SIBIRICA I.) | 2011 |
|
RU2479992C1 |
METHOD OF MICROCLONAL REPRODUCTION OF ASPEN HYBRIDES | 0 |
|
SU1581741A1 |
BREEDING OF PLUMEPOPPY CORDATE (MACLEAYA CORDATA (WILLD.) R.BR.) (VARIANTS) | 2003 |
|
RU2264084C2 |
METHOD FOR GROWING PLANT RAW MATERIALS OF SIBERIAN IRIS (IRIS SIBIRICA L.) WITH A GIVEN CONTENT OF EXTRACTIVES | 2021 |
|
RU2773523C1 |
Authors
Dates
2015-04-10—Published
2013-08-14—Filed