METHOD FOR DETERMINATION OF NATURAL AMINO ACIDS IN FOOD PRODUCTS PROTEIN COMPOSITION Russian patent published in 2014 - IPC G01N33/02 G01N1/18 C07K7/08 

FIELD: food industry.

SUBSTANCE: method envisages the sample acid hydrolysis, the hydrolysate filtration and chromatographic separation with subsequent automatic identification and quantitative evaluation of amino acids content using an automatic analyser. The invention allows to determine amino acids in the food product proteins composition with amino acids content equal to nearly 0.1-3.5 g/100 g of the product (1.5-17 g/100 g of protein) with application of sequential elution of amino acids with a buffer solutions mixture and simultaneous detection of the components at two wave lengths being 440 and 570 nm.

EFFECT: acceleration of the process of amino acids isolation from the food product and determination accuracy enhancement due to losses decrease and highly sensitive material application.

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