FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry. Method of producing preparation of protein from sample of mixture, containing protein is described, which is target of extraction and at least one HCP. Method involves stage of analysis by Raman spectroscopy of said sample of mixture; bringing of said sample of mixture into contact with affinity chromatographic resin in chromatographic separation with simulated moving bed; collection of chromatographic sample; and performing analysis of Raman spectroscopy of said chromatographic sample for identification of preparation targeted for extraction with reduced HCP, where said protein targeted for extraction represents antibody or its antigen-binding part.
EFFECT: invention extends range of products for purification of proteins.
9 cl, 41 dwg, 4 tbl, 11 ex
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