METHOD OF PRODUCING ANALYTICAL TEST SYSTEM FOR MULTIPLEX IDENTIFICATION AND QUANTITATIVE MEASUREMENT OF CONTENT OF PROTEINS OF INTEREST IN BIOLOGICAL SAMPLE BY CONTENT OF CORRESPONDING PROTEOTIPC MARKER PEPTIDES Russian patent published in 2016 - IPC G01N33/00 

FIELD: biochemistry.

SUBSTANCE: invention relates to a method of producing an analytical test system (MRM-test) for multiplex identification and quantitative measurement of content of protein of interest in a biological sample based on content of corresponding proteotypic marker peptides, including detection of unique for protein proteotypic marker peptide sequences; extraction of at least two proteotypic marker peptide sequences of protein; prediction of peptide fragments; prediction of MRM-test in form of a list of marker peptides, their fragments and best detection parameters; synthetic peptide marker; determination of profile transitions of synthetic marker peptides; optimising MRM-test in accordance with obtained profiles; cleaning peptides; preparation of a biological sample; identification of protein in a biological sample with weir of synthetic peptides; determination of retention time of marker peptides with application of values determined in MRM tests; conducting multiplex calibration measurements; quantitative measurement of content of marker peptides in biological sample; and a judgment on content of protein of interest in a biological sample.

EFFECT: invention provides high sensitivity and reduced time for determining proteotypic peptides and target protein of interest.

4 cl, 9 dwg, 1 tbl, 7 ex