METHOD FOR MEASURING BLOOD 2,4-DICHLORPHENOL BY GAS CHROMATOGRAPHY ANALYSIS Russian patent published in 2014 - IPC G01N33/50 G01N30/02 

FIELD: medicine.

SUBSTANCE: method involves blood sampling, 2,4-dichlorophenol extraction by an organic extractant from the above sample and measurement by gas chromatography analysis with using a calibration curve; before extraction, the blood sample is acidified with an oxalic acid solution to pH 2-3; the organic extractant for the extraction is presented by toluene; further, the prepared extract is added with a bromating agent and a water-thinned sulphuric acid in volume ratio of water : concentrated sulphuric acid as 3:1 respectively; the extract is brominated for 5 minutes that is followed by neutralising a bromine excess with sodium sulphite; the prepared brominated extract is centrifuged to separate toluene, and acetylated with trifluoroacetic anhydride in the pyridine medium for 5 minutes; the blood sample, the organic extractant toluene, the bromating agent, trifluoroacetic anhydride and pyridine are taken in the following volume ratio of 1:0.5:0.4:0.02:0.02 respectively.

EFFECT: simplifying the sample preparation stage in a combination with higher sensitivity.

5 cl, 6 tbl, 1 ex