TEST STRIP FOR HIGHLY SENSITIVE IMMUNOCHROMATOGRAPHIC ANALYSIS Russian patent published in 2014 - IPC G01N33/53 

FIELD: chemistry.

SUBSTANCE: method is based on a contact of a membrane test strip with an analysed liquid sample and initiated by the said contact movement on the test strip membranes of reagents, contained in the sample or applied on the membrane and forming immune complexes in membrane pores or its surface in the course of interactions. Intensity of coloration of a mark, which binds in an analytic zone, is detected visually. The claimed system is intended for immunochromatographic determination of antibodies or antigens in liquid samples. A characteristic feature of the claimed method of carrying out an immunochromatographic analysis consists in the following: a multimembrane composite of the test strip contains an additional membrane with a substance applied on it substance, which dissolves in water slowly, for instance a bovine serum albumin (BSA), which is attached to the test strip immediately after the membrane with the applied colloid conjugate. The front of the liquid sample, passing the membrane with colloidal gold, conjugated with a reagent for analite binding, stops, after getting on the membrane with BSA, until BSA is dissolved. During this time the sample is incubated with the colloidal conjugate, which approaches a reaction of analite binding to an equilibrium position.

EFFECT: reduction of detection limit of the analysis.

2 dwg, 1 ex