FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology and specifically to a method of conducting immunochromatographic assay. The method includes detecting colloidal marker-coloured specific antigen-antibody complexes on the surface of a working membrane. The method employs reversible immobilisation of immunoreagents in analytical zones of the working membrane, for which immunospecific reagents are conjugated with magnetic nanosized carriers and held in the analytical zones with application of a magnetic field, which enables to eliminate limitations on immobilisation of reagents on working membranes and perform a specific reaction outside the testing strip.
EFFECT: present invention provides the best detection and evaluation of results, and improves sensitivity of the method.
2 dwg, 1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD TO REDUCE LIMIT OF DETECTION OF IMMUNOCHROMATOGRAPHIC METHODS TO CONTROL CONTENT OF LOW-MOLECULAR COMPOUNDS | 2011 |
|
RU2497126C2 |
| METHOD FOR HIGHLY SENSITIVE COMPETITIVE IMMUNOCHROMATOGRAPHIC ANALYSIS | 2022 |
|
RU2789545C1 |
| UNIVERSAL TEST STRIP FOR SELECTIVE DETERMINATION OF MYCOTOXINS BY IMMUNOCHROMATOGRAPHY | 2021 |
|
RU2806175C2 |
| METHOD FOR PERFORMING IMMUNOCHROMATOGRAPHIC ASSAY WITH HIGH DEGREE OF MARKER DETECTION | 2015 |
|
RU2623075C1 |
| METHOD OF IMMUNOCHROMATOGRAPHIC SERODIAGNOGY WITH SEQUENTIAL ADDITION OF REAGENTS | 2020 |
|
RU2741199C1 |
| METHOD OF IMMUNOCHROMATOGRAPHIC ASSAY OF MILK AND DAIRY PRODUCTS FOR ANTIBIOTICS | 2009 |
|
RU2406090C2 |
| METHOD FOR IMMUNOCHROMATOGRAPHIC ANALYSIS FOR SERODIAGNOSTICS WITH A COMBINED ANTIBODY BINDING PATTERN | 2020 |
|
RU2753237C1 |
| TEST STRIP FOR HIGHLY SENSITIVE IMMUNOCHROMATOGRAPHIC ANALYSIS | 2013 |
|
RU2523393C1 |
| METHOD FOR HIGHLY SENSITIVE IMMUNOCHROMATOGRAPHIC ANALYSIS WITH DOUBLE COMPETITION | 2020 |
|
RU2748901C1 |
| VISCOSITY OF ENVIRONMENT AS TOOL TO CONTROL END OF IMMUNOCHROMATOGRAPHIC TEST SYSTEMS DETECTION | 2016 |
|
RU2645907C1 |
