METHOD OF PREDICTING DEVELOPMENT OF IMMUNE DISORDERS UNDER IMPACT OF STRONTIUM WITH APPLICATION OF IMMUNOGENETIC CRITERIA Russian patent published in 2015 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: to predict the development of immune disorders in patients living on territories with the high content of strontium in the environment, sampling of blood in the patients from the territory with the higher content of strontium in objects of the environment was carried out. In the said sample determined is the content of strontium in the blood samples of the patients, with the content of strontium higher than the background value, the content of lymphocytes CD8⁺ is determined. After that, a separated fraction of CD8⁺ lymphocytes is divided into two parts, one of which is subjected to incubation with strontium, and the second is used as a control one, with the incubation being realised by the introduction of 10 mg/l of strontium into the CD8⁺ sample. Then, an amount of ribonucleic acid RNA is determined in both samples of CD8⁺ lymphocytes by its isolation from separated CD8⁺ cells, performed is a reaction of the reverse RNA transcription into deoxyribonucleic acid DNA, in which the quantity of the alpha defensin gene is determined. In determination the gene of GAPDH household is used as internal control. After that the level of the relative expression of the alpha defensin gene is calculated, taking as a spontaneous expression the level referring to the control CD8⁺ sample, with the level, referring to the CD8⁺ sample, into which strontium was introduced, being taken as the induced expression. A conclusion about immune disorders in the patient, associated with the impact of strontium, is made by the index of the expression, which equals the ratio of the induced expression to the spontaneous expression. If the said index is lower than 0.7, the development of an immunodeficiency state is predicted, and if the index is higher than 3, the development of a proliferative status in the immune system, on the condition of the simultaneous detection of higher than 1.5-fold exceedance of strontium in blood in comparison with the background one.

EFFECT: prediction of the immune disorders, associated with the impact of strontium, due to the application of the immunogenetic criteria.

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