RECOMBINANT PSEUDO-ADENOVIRUS PARTICLE BASED ON GENOME OF HUMAN ADENOVIRUS OF SEROTYPE 5, PRODUCING INFLUENZA HEMAGGLUTININ OF STRAIN A/Brisbane/59/2007(H1N1) AND METHOD OF ITS USE Russian patent published in 2014 - IPC C12N7/01 C12N15/09 C12N15/44 A61K39/145 

FIELD: biotechnology.

SUBSTANCE: recombinant pseudo-adenovirus particle based on the genome of human adenovirus of serotype 5 and the method of its use are characterised. The presented particle comprises an expressing cassette with the insert of the hemagglutinin gene of influenza virus, at that the hemagglutinin gene of influenza virus was used as hemagglutinin of strain A/Brisbane/59/2007(H1N1) with the nucleotide sequence preliminary optimised for expression in human cells, presented in SEQ ID N0:2. The said haemagglutinin gene of influenza virus is cloned into an expression cassette comprising the signal of polyadenylation SV40 under control of the cytomegalovirus promoter. The presented invention can be used to induce specific immunity against influenza virus of A subtype H1 and H5 when administered to a subject in an effective amount, by providing enhanced expression of the recombinant hemagglutinin of influenza virus A/Brisbane/59/2007 (H1N1).

EFFECT: increased efficiency of connection use.

6 cl, 9 dwg, 1 tbl, 4 ex