KIT OF REAGENTS FOR SIMULTANEOUS OR SEPARATE IMMUNOHISTOCHEMICAL DETECTION OF OESTROGEN, PROGESTERONE AND HER2 RECEPTORS IN DIAGNOSING BREAST CANCER Russian patent published in 2014 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: kit of reagents contains a solution for providing an antigen bioavailability, a blocking solution of horseradish peroxidase, a protein blocking solution, a washing solution, a solution of primary HER2 receptor antibodies, a solution of primary oestrogen receptor antibodies, a solution of primary progesterone receptor antibodies, a solution of anti-species secondary antibodies to primary antibodies, a solution of streptavidin - horseradish peroxidase, diamine benzidine, a substrate solution, a negative reference sample, three positive reference samples of breast cancer for detecting HER2, oestrogen receptors, progesterone receptors. The kit of reagents according to the invention possesses higher sensitivity and specificity. The invention may be used in medical laboratories and research establishments.

EFFECT: possibility to detect oestrogen and progesterone receptors, as well as HER2 receptors either simultaneously, or separately.

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