METHOD OF PRODUCING PREPARATIVE QUANTITIES OF PHLOEM-RESTRICTED VIRAL ANTIGENS Russian patent published in 2014 - IPC C12N15/10 

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry, particularly a method of producing preparative quantities of a viral antigen - potato leaf roll virus (PLRV) protein, in chimeric viral particles imitating PLRV virions, the method comprising producing recombinant DNA containing cDNA of turnip vein-clearing virus (TVCV) which belongs to tobamoviruses, where the TVCV cDNA is under the control of a plant promoter and there is a transcription terminator at its end, and the TVCV coat protein gene is substituted with a PLRV major coat protein gene, which includes producing agrobacterial vector (plasmid) pCambia 1300, which is capable of being embedded into the host-plant gene and cause system infection, transferring the formed recombinant DNA into the agrobacterial vector pCambia, transforming a Nicotiana benthamian host-plant with the agrobacterial vector pCambia, reproduction of the chimeric virus, consisting of viral DNA, in the Nicotiana benthamian host-plant, separating the chimeric virus containing TVCV cDNA, the coat gene of which is substituted with the a PLRV major coat protein gene, from infected leaves of the Nicotiana benthamian host-plant. Disclosed is a method of producing antiserum against PLRV capsid protein, which includes immunising animals with viral particles obtained using said method.

EFFECT: invention enables to obtain 2-4 mg of viral chimera from 11 g of infected leaves.

2 cl, 7 dwg, 1 tbl