FIELD: biotechnologies.
SUBSTANCE: homogenisation of tissues of an infected plant is performed in neutral buffer solution based on 2-[4-(2-hydroxyethyl)piperazin-1-il]ethanesulphonic acid (HEPES) in concentration of 0.02-0.03 M with addition of inhibitors of plant ferments of iodine-sodium acetate and phenilmethylsulfonyl fluoride. Saccharose is added. Obtained extract is clarified. Clarified extract is treated with 4 - 6% Triton X-100 to separate viral particles from cell components. Viral particles are separated from clarified extract by differential centrifugation or ultracentrifugation.
EFFECT: method allows increasing yield of viral particles cleaned from plant impurities from 100 g of fresh leaves by 2-3 times.
4 dwg, 4 ex
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