METHOD OF PRODUCTION OF BACTERIOPHAGE Russian patent published in 2014 - IPC C12N7/00 A61K35/76 

FIELD: biotechnology.

SUBSTANCE: method of production of bacteriophage comprises: inoculation with the bacterial culture of the host strain in the titre of 108-109 CFU/ml in the vessel for culturing on the slant having a layer thickness from 10 mm to 25 mm, culturing for 3-3.5 hours at optimum temperature for growth of the host strain culture, then on the resulting lawn of culture of the host strain the stock bacteriophage is inoculated in the titre 10⁵-10⁶ CFU/ml and cultured for 13-15 hours at the optimum temperature and the thickness of the air layer over the surface of the solid medium of 25 mm to 40 mm. the phage lysate is prepared and sucked into a sterile container, chloroform is added, incubated for 30-45 minutes with continuous shuttling. The supernatant is centrifuged and sterilised by filtration through the filter with a pore diameter of 0.2-0.22 microns.

EFFECT: solution provides the universal possibility of production of phage lysate with use of different bacteriophages, stability of bacteriophage titre in phage lysate and the possibility of its use in the food industry.

3 cl, 3 ex