METHOD FOR INDUCED DNA DAMAGE IN INDIVIDUAL NON-DIVIDING NUCLEATED CELLS Russian patent published in 2014 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: blood cell sample is used to prepare slides of several layers of agarose one of which contains individual non-dividing nucleated cells. Damaging the cell DNA is carried out by phosphate-buffer saline with pH 7.4-7.5 with ozone-oxygen mixture flown through preliminary for 5 min with the ozone concentration of 500-1000 mcg/l. The cells are broken down at pH=10; the DNA is denatured by placing the slides into an alkaline solution at pH>13; the damaged DNAs of the broken down cells are subject to an electrophoresis in the solution of pH>13 at voltage 27 V, current intensity 260-270 mA, field density 2.0 V/cm. The fluorescent stained damaged DNAs are recorded by photographing, software-processed; a percentage ratio of the damage DNAs to their total number is calculated; if the derived ratio is more than 10%, the induced DNA damages are stated.

EFFECT: method enables diagnosing malignant new growths, selecting drug preparations and geriatric protectors in radio-, chemo- and ozone therapy.

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