FIELD: medicine.
SUBSTANCE: bacteria freely immobilised on a slide are processed with a lysis solution containing an ionogenic protein-denaturing detergent and EDTA. Further, DNA-nucleoid is dry heating and microwave incubation stabilised. DNA is stained by high-sensitivity DNA-specific fluorochrome, in conclusion DNA integrity is evaluated. Offered is a kit for evaluating the DNA bacteria integrity which comprises all the components required.
EFFECT: invention can be used for determining the bacterial DNA fragmentation levels by a precise express technique.
16 cl, 11 dwg, 5 tbl, 9 ex
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