FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology and can be used for determining integrity of chromatin/DNA in spermatozoa of animals. A spermatozoa-containing sample is treated with a DNA denaturing solution. To extract nuclear proteins, treatment is done in a single stage using a lysing solution, which is free from protein denaturing detergents. Integrity of chromatin/DNA of spermatozoa is visually determined by colouring based on measuring size of halo spermatozoa.
EFFECT: invention simplifies and increases accuracy of determining integrity of chromatin/DNA in spermatozoa of animals.
14 cl, 5 dwg, 10 tbl, 8 ex
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