METHOD OF DETECTING MICROORGANISM OF SPECIES VIBRIO PARAHAEMOLYTICUS Russian patent published in 2014 - IPC C12N7/00 G01N33/50 C12R1/63 

Abstract RU 2531236 C1

FIELD: biotechnology.

SUBSTANCE: method comprises inoculation of the test strain on the lawn of the culture, applying a drop of diagnostic phage preparation in centre of the cup, holding at the temperature of 37°C for 20-24 hours and confirmation of its belonging to the microorganisms of the species V. parahaemolyticus in the presence of lysis. At that the diagnostic phage preparation is prepared by mixing phages FC-44 and FC-46, prepared based on the strain Vibrio parahaemolyticus KM-97, in the ratio of 1:1 in the titre n·108 PFU/ml.

EFFECT: present invention enables to accelerate the identification of strains and can be used in the laboratory diagnostics of acute intestinal infections caused by this microorganism.

2 cl, 1 tbl, 3 ex

Similar patents RU2531236C1

Title Year Author Number
METHOD FOR IDENTIFICATION OF CHOLERA VIBRIO O1 SEROGROUPS OF BIOVARS CLASSICAL AND EL TOR 2019
  • Gaevskaya Natalya Evgenevna
  • Anoprienko Anna Olegovna
  • Pogozhova Marina Pavlovna
  • Tyurina Anna Vladimirovna
RU2729575C1
METHOD FOR DIFFERENTIATION OF PARAHEMOLYTIC VIBRIA OF III, IV, V MORPHOGROUPS FROM FILAMENTOUS BACTERIOPHAGES OF I MORPHOGROUP 2005
  • Kudrjakova Tat'Jana Aleksandrovna
  • Gaevskaja Natal'Ja Evgen'Evna
  • Makedonova Ljudmila Dmitrievna
  • Kachkina Galina Vital'Evna
  • Sajamov Sergej Rantovich
RU2290445C1
VIBRIO METSCHNIKOVII BACTERIA STRAIN AS INDICATOR CULTURE FOR SPECIFIC PHAGE DETECTION 2006
  • Kudrjakova Tat'Jana Aleksandrovna
  • Terent'Ev Aleksandr Nikolaevich
  • Makedonova Ljudmila Dmitrievna
  • Kachkina Galina Vital'Evna
  • Alieva Anna Aleksandrovna
RU2332453C1
METHOD FOR EXTRACTION AND DIFFERENTIATION OF PHAGE VIBRIO MIMICUS 2008
  • Kudrjakova Tat'Jana Aleksandrovna
  • Makedonova Ljudmila Dmitrievna
  • Kachkina Galina Vital'Evna
  • Avdeeva Elena Petrovna
  • Sajamov Sergej Rantovich
RU2375437C1
METHOD FOR IDENTIFYING HALOPHILIC VIBRIOS 0
  • Golkovskij Gidalij Moiseevich
  • Libinzon Anna Evelevna
  • Kirdeev Vitalij Kuzmich
SU1124031A1
Yersinia enterocolitica BACTERIA STRAIN USED AS INDICATOR CULTURE FOR DETECTING MODERATE BACTERIOPHAGES OF LYSOGENIC STRAINS OF O1, O3, O12 SEROVARS 2010
  • Kudrjakova Tat'Jana Aleksandrovna
  • Makedonova Ljudmila Dmitrievna
  • Kachkina Galina Vital'Evna
  • Goncharenko Elena Vladimirovna
RU2425872C1
STOCK VIBRIO CHOLERAE ELTOR 692,USED FOR DETECTING CHOLERA TEMPERATURE 123-TYPE PHAGES 0
  • Ostroumova Nonna Mikhajlovna
  • Moroz Valentina Petrovna
  • Korovkina Galina Ivanovna
  • Shmerkevich Dina Leonidovna
SU1216198A1
METHOD FOR INTRASPECIES DIFFERENTIATION OF Vibrio cholerae O139 2004
  • Kudrjakova Tat'Jana Aleksandrovna
  • Gaevskaja Natal'Ja Evgen'Evna
  • Makedonova Ljudmila Dmitrievna
  • Kachkina Galina Vital'Evna
RU2268942C1
STRAIN OF VIBRIO CHOLERAE 042 BACTERIA USABLE AS INDICATOR CULTURE FOR IDENTIFYING MODERATE FAGE AND ITS VIRULENT MUTANTS 0
  • Ostroumova N.M.
  • Moroz V.P.
  • Zinina O.S.
  • Kudryakova T.A.
  • Ushakova I.E.
  • Konnov N.P.
SU1391090A1
STRAIN OF VIBRIO CHOLERAE BACTERIA AS SOURCE OF MILD FACE 0
  • Ostroumova Nonna Mikhajlovna
  • Moroz Valentina Petrovna
  • Korovkina Galina Ivanovna
  • Uspenskaya Lyudmila Vasilevna
  • Karavaeva Tatyana Borisovna
  • Konnov Nikolaj Pavlovich
SU1454849A1

RU 2 531 236 C1

Authors

Kudrjakova Tat'Jana Aleksandrovna

Gaevskaja Natal'Ja Evgen'Evna

Makedonova Ljudmila Dmitrievna

Kachkina Galina Vital'Evna

Dates

2014-10-20Published

2013-03-19Filed