FIELD: biotechnologies.
SUBSTANCE: method to cultivate sublimated strains of microorganisms includes introduction of a growth stimulant and a source of carbon into a dense nutrient medium with subsequent seeding of microorganism cells, incubation of seeds and accounting of viable microbial cells. Sublimated strains of microorganisms include Yersinia pestis EV, Escherichia coli C600, Bacillus anthracis "СТИ", Vibrio cholerae NAG 504, Brucella abortus 19BA. The growth stimulant and carbon source is 96% ethyl alcohol in the amount of 1.0-2.0% of the medium volume. If necessary, they add gentian violet in concentration of 1:100000.
EFFECT: improved yield of viable strain cells on dense nutrient media.
1 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF MICROORGANISM CULTURING | 1999 |
|
RU2163265C2 |
| NUTRIENT BASE PRODUCTION METHOD AND NUTRIENT BASE FOR YERSINIA AND VIBRIO MICROORGANISMS FERMENTATION | 2007 |
|
RU2360962C2 |
| METHOD FOR PRODUCTION OF FETAL MICROORGANISM GROWTH STIMULATOR | 2004 |
|
RU2283347C2 |
| LIQUID NUTRIENT MEDIUM FOR DEEP CULTIVATION OF VACCINE STRAIN OF PLAGUE MICROBE Y. PESTIS EV | 2021 |
|
RU2757428C1 |
| METHOD FOR PRODUCING PATHOGENIC BIOLOGICAL AGENT SIMULATORS | 2015 |
|
RU2607369C1 |
| LIQUID NUTRITIONAL MEDIUM FOR CULTIVATION AND DISCHARGE OF BIOMASS OF VACCINE STRAIN OF PLAGUE MICROBE (YERSINIA PESTIS EV) | 2020 |
|
RU2745504C1 |
| NUTRIENT MEDIUM FOR CULTIVATION OF YERSINIA PESTIS EV | 2018 |
|
RU2708029C1 |
| METHOD OF ISOLATING PLAGUE MICROBE BY BACTERIOLOGICAL METHOD FROM INVESTIGATED MATERIAL (VERSIONS) | 2007 |
|
RU2353654C1 |
| METHOD FOR INCREASING IMMUNOGENICITY OF VACCINE STRAIN OF PLAGUE MICROBE | 2019 |
|
RU2727258C1 |
| DENSE NUTRIENT MEDIUM FOR CULTIVATION AND COLLECTION OF BIOMASS OF VACCINE STRAIN OF PLAGUE MICROBE YERSINIA PESTIS EV | 2018 |
|
RU2702174C1 |
